OAR@UM Collection:https://www.um.edu.mt/library/oar/handle/123456789/332122026-07-17T04:01:09Z2026-07-17T04:01:09ZInvestigating pancreatic α-amylase in gastric juicehttps://www.um.edu.mt/library/oar/handle/123456789/333142020-11-09T09:09:19Z2008-01-01T00:00:00ZTitle: Investigating pancreatic α-amylase in gastric juice
Abstract: The aims of the study were to develop a method that quantitatively measures P-AM in
gastric juice; to differentiate between P-AM and S-AM in gastric juice; to investigate
the effect of pH, temperature and time on P-AM detection in gastric juice; to
qualitatively analyse P-AM in gastric juice; to investigate the effect of PPI treatment on
P-AM.
P-AM and AMYL activities were measured at room temperature and on ice. A P-AM
pH curve was obtained by testing P-AM activity at different pH values. Different buffer
to gastric juice ratio were used to measure P-AM and AMYL activities for an optimum
ratio. P-AM and AMYL activities were assayed using the Reflotron® and Phadebas®
Amylase test and calibration curves were obtained. Gastric juice from thirteen patient
samples on PPT treatment and from four controls was tested for P-AM activity.
There was a significant difference between P-AM activity at different time intervals
with a P-value of 0.00679 (P<0.05). There was no significant difference between the
effect of temperature and time with a P-value of 0.384 (P>0.05). The P-AM calibration
curves show lower measured P-AM activity than standard values. A significant
correlation was noted with a correlation co-efficient of 0.9998 (P<0.05) using the
Reflotron ®.
The Reflotron® and the Phadebas® Amylase test can quantitatively analyse P-AM and
AMYL activities in gastric juice whereas Iso-Electrofocusing can be used for qualitative
analysis. The Hitachi 917 quantitatively analysed AMYL activity and the Agarose
method quantitatively analysed P-AM activity in gastric juice. Denaturing of P-AM
occurs at a pH < 4.00 and> 10.00. The optimum buffer to gastric juice ratio is 55:45.
The time taken from collection to analysis of P-AM activity is critical using the
Reflotron® which is not influenced by storage temperature. Hyperamylasemia was
present in gastric juice of patients on PPI treatment. No P-AM was found present in the
four controls.
Description: M.PHIL.2008-01-01T00:00:00Z