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dc.contributor.authorAquilina, A.
dc.contributor.authorFarrugia, Claude
dc.contributor.authorSinagra, Emmanuel
dc.identifier.citationAquilina, A., Farrugia, C., & Sinagra, E. (2002). The effect of sodium chloride on ethanolic fractionation of dilute gelatin solutions. 4th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, Florence. 1-2.en_GB
dc.description.abstractGelatin is the denaturation product of the protein collagen, the main constituent of animal skin, bone, and connective tissue. The conversion of collagen to gelatin results in a heterogeneous product with a broad molecular weight profile (MWP), which is important in determining the behaviour of the protein in solution. Addition of successive increments of a non-solvent, such as ethanol, to gelatin solutions causes the Florey- Huggins solvent-protein interaction parameter, χ, of the system to successively exceed the critical value for the different molecular weight fractions, causing progressive desolvation of the polymer. When sufficient solvent molecules are removed, the gelatin molecules begin to aggregate, resulting in phase separation, and forming a coacervate or, if sufficient desolvation occurs, a precipitate. Modification of the net charge of the protein molecules, by adjusting the solution pH to values ranging about the iso-electric point (IEP), influences the degree of interaction between the different molecular weight fractions, and hence the response of the protein to non-solvent.1 It can be hypothesised that alteration of the molecular charge intensity by changes in the ionic strength of the solution would affect the overall response of the protein. The objective of this work was to determine the effect of dilute NaCl concentrations on the response of B225 and B75 gelatins to the non-solvent ethanol at different pH’s.en_GB
dc.subjectHydrogen-ion concentrationen_GB
dc.subjectSeparation (Technology)en_GB
dc.titleThe effect of sodium chloride on ethanolic fractionation of dilute gelatin solutionsen_GB
dc.rights.holderThe copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder.en_GB
dc.bibliographicCitation.conferencename4th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technologyen_GB
dc.bibliographicCitation.conferenceplaceFlorence, Italy, 8-11/04/2002en_GB
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