Please use this identifier to cite or link to this item: https://www.um.edu.mt/library/oar/handle/123456789/22403
Title: Role(s) of the 5-HT2C receptor in the development of maximal dentate activation in the hippocampus of anesthetized rats
Authors: Orban, Gergely
Bombardi, Cristiano
Marino Gammazza, Antonella
Colangeli, Roberto
Pierucci, Massimo
Pomara, Cristoforo
Pessia, Mauro
Bucchieri, Fabio
Arcangelo, Benigno
Smolders, Ilse J.
De Deurwaerdere, Philippe
Di Giovanni, Giuseppe
Keywords: Receptor, Serotonin, 5-HT2C
Depression, Mental
Dentate gyrus
Temporal lobe epilepsy
Issue Date: 2014
Publisher: Wiley-Blackwell Publishing Ltd.
Citation: Orban, G., Bombardi, C., Marino Gammazza, A., Colangeli, R., Pierucci, M., Pomara, C., Pessoa, M., Bucchieri, F.,…Di Giovanni, G. (2014). Role(s) of the 5-HT2C receptor in the development of maximal dentate activation in the hippocampus of anesthetized rats. CNS Neuroscience & Therapeutics, 20(7), 651-661.
Abstract: Aims: Substantial evidence indicates that 5-HT2C receptors are involved in the control of neuronal network excitability and in seizure pathophysiology. Here, we have addressed the relatively unexplored relationship between temporal lobe epilepsy (TLE), the most frequent type of intractable epilepsy, and 5-HT2CRs. Methods: In this study, we investigated this issue using a model of partial complex (limbic) seizures in urethane-anesthetized rat, based on the phenomenon of maximal dentate activation (MDA) using 5-HT2C compounds, electrophysiology, immunohistochemistry, and western blotting techniques. Results: The 5-HT2C agonists mCPP (1 mg/kg, i.p) and lorcaserin (3 mg/kg, i.p), but not RO60-0175 (1–3 mg/kg i.p.), were antiepileptogenic reducing the MDA response duration. The selective 5-HT2C antagonist SB242084 (2 mg/kg, i.p) unveiled antiepileptogenic effects of RO60- 0175 (3 mg/kg, i.p) but did not alter those induced by mCPP and lorcaserin. Compared with control rats, electrically stimulated rats showed an increase in glutamic acid decarboxylase levels and a heterogeneous decrease in 5-HT2CR immunoreactivity in different hippocampal areas. Conclusions: In our animal model of TLE, mCPP and lorcaserin were anticonvulsant; likely acting on receptor subtypes other than 5-HT2C. Epileptogenesis induced early adaptive changes and reorganization in the 5-HT2CR and GABA systems.
URI: https://www.um.edu.mt/library/oar//handle/123456789/22403
Appears in Collections:Scholarly Works - FacM&SPB

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