Please use this identifier to cite or link to this item:
Title: Expression of CD44 and integrins in bronchial mucosa of normal and mildly asthmatic subjects
Authors: Peroni, Diego Giampietro
Djukanovic, Ratko
Bradding, Peter B.
Feather, Iain H.
Montefort, Stephen
Howarth, Peter Hugo
Jones, David B.
Holgate, Stephen T.
Keywords: Cell adhesion molecules
Issue Date: 1996
Publisher: ERS
Citation: Peroni, D., Djukanovic, R., Bradding, P., Feather, I., Montefort, S., Howarth, P.,...Holgate, S. (1996). Expression of CD44 and integrins in bronchial mucosa of normal and mildly asthmatic subjects. The European Respiratory Journal, 9(11), 2236-2242.
Abstract: We have investigated the expression of cell surface markers and leucocyte cell adhesion molecules by immunohistochemistry in bronchial biopsies from 10 mild atopic asthmatics and 8 normal, nonatopic subjects. Significantly increased numbers of eosinophils (p<0.01) were evident in the bronchial submucosa of asthmatic subjects. In epithelium there were more CD44+ (p<0.02) and lymphocyte function-associated antigen-1 (LFA-1)+ (p<0.06) leucocytes in asthmatics than in normal subjects. Bronchial epithelial cells stained positively with anti-CD44 monoclonal antibodies (moAb) in both groups; however, when the staining was expressed as percentage of the total basement membrane, a considerable and highly significant increase was observed in the asthmatics (median 80 vs 22%, p=0.003). Few leucocytes were positive for very late activation antigen (VLA)-1, VLA-2 and VLA-4. The moAb for VLA-6 stained the basement membrane of the bronchial epithelium; while intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were constitutively expressed in endothelium. A positive correlation was found between LFA-1+ cells and activated eosinophils (EG2+) in the submucosa (p<0.005; r(s)=0.80). We conclude that even in mild asthma there is evidence of increased expression of cell surface ligands, and suggest that adhesive mechanisms play a role both in cell recruitment and disease activity.
Appears in Collections:Scholarly Works - FacM&SMed

Files in This Item:
File Description SizeFormat 
Expression of CD44 and integrins in bronchial mucosa__.pdf202.82 kBAdobe PDFView/Open

Items in OAR@UM are protected by copyright, with all rights reserved, unless otherwise indicated.