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|Title:||Cloned prokaryotic iron superoxide dismutase protects yeast cells against oxidative stress depending on mitochondrial location|
Galea Agius, Dorita
Bannister, William H.
|Citation:||Balzan, R., Agius, D. R., & Bannister, W. H. (1999). Cloned prokaryotic iron superoxide dismutase protects yeast cells against oxidative stress depending on mitochondrial location. Biochemical and Biophysical Research Communications, 256(1), 63-67.|
|Abstract:||Superoxide dismutase (SOD) is considered to be the first line of defense against oxygen toxicity. It exists as a family of three metalloproteins with copper,zinc (Cu,ZnSOD), manganese (MnSOD), and iron (FeSOD) forms. In this work, we have targetedEscherichia coliFeSOD to the mitochondrial intermembrane space (IMS) of yeast cells deficient in mitochondrial MnSOD. Our results show that FeSOD in the IMS increases the growth rate of the cells growing in minimal medium in air but does not protect the MnSOD-deficient yeast cells when exposed to induced oxidative stress. Cloned FeSOD must be targeted to the mitochondrial matrix to protect the cells from both physiological and induced oxidative stress. This confirms that the superoxide radical is mainly generated on the matrix side of the inner mitochondrial membrane of yeast cells, without excluding its potential appearance in the mitochondrial IMS where its elimination by SOD is beneficial to the cells.|
|Appears in Collections:||Scholarly Works - FacM&SPB|
Scholarly Works - JCBio
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|Galea, Dolores R. _Cloned Prokaryotic Iron Superoxide Dismutase Protects.pdf||470.74 kB||Adobe PDF||View/Open|
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