Please use this identifier to cite or link to this item: https://www.um.edu.mt/library/oar/handle/123456789/24995
Title: Cloned prokaryotic iron superoxide dismutase protects yeast cells against oxidative stress depending on mitochondrial location
Authors: Balzan, Rena
Galea Agius, Dorita
Bannister, William H.
Keywords: Superoxide dismutase
Oxidative stress
Saccharomyces cerevisiae
Superoxide dismutase
Issue Date: 1999
Publisher: Elsevier Inc.
Citation: Balzan, R., Agius, D. R., & Bannister, W. H. (1999). Cloned prokaryotic iron superoxide dismutase protects yeast cells against oxidative stress depending on mitochondrial location. Biochemical and Biophysical Research Communications, 256(1), 63-67.
Abstract: Superoxide dismutase (SOD) is considered to be the first line of defense against oxygen toxicity. It exists as a family of three metalloproteins with copper,zinc (Cu,ZnSOD), manganese (MnSOD), and iron (FeSOD) forms. In this work, we have targetedEscherichia coliFeSOD to the mitochondrial intermembrane space (IMS) of yeast cells deficient in mitochondrial MnSOD. Our results show that FeSOD in the IMS increases the growth rate of the cells growing in minimal medium in air but does not protect the MnSOD-deficient yeast cells when exposed to induced oxidative stress. Cloned FeSOD must be targeted to the mitochondrial matrix to protect the cells from both physiological and induced oxidative stress. This confirms that the superoxide radical is mainly generated on the matrix side of the inner mitochondrial membrane of yeast cells, without excluding its potential appearance in the mitochondrial IMS where its elimination by SOD is beneficial to the cells.
URI: https://www.um.edu.mt/library/oar//handle/123456789/24995
Appears in Collections:Scholarly Works - FacM&SPB
Scholarly Works - JCBio

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