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DC Field | Value | Language |
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dc.contributor.author | Fuhr, Josephy E. | - |
dc.contributor.author | Bamberger, Elena G. | - |
dc.contributor.author | Lozzio, Carmen B. | - |
dc.contributor.author | Lozzio, Bismarck B. | - |
dc.contributor.author | Felice, Alex | - |
dc.contributor.author | Altay, Gultekin | - |
dc.contributor.author | Webber, Brooke B. | - |
dc.contributor.author | Reese, A. L. | - |
dc.contributor.author | Mayson, Sylvia M. | - |
dc.contributor.author | Huisman, Titus Hendrik Jan | - |
dc.date.accessioned | 2018-03-16T13:34:44Z | - |
dc.date.available | 2018-03-16T13:34:44Z | - |
dc.date.issued | 1982 | - |
dc.identifier.citation | Fuhr, J. E., Bamberger, E., Lozzio, C. B., Lozzio, B. B., Felice, A. E., Altay, G.,...Huisman, T. H. J. (1982). Identification and quantitation of embryonic and three types of fetal hemoglobin produced on induction of the human pluripotent leukemia cell line K‐562 with hemin. American Journal of Hematology, 12(1), 1-12. | en_GB |
dc.identifier.uri | https://www.um.edu.mt/library/oar//handle/123456789/28038 | - |
dc.description.abstract | The hemoglobins synthesized by the pluripotent K‐562 leukemia cell line of human origin after induction with hemin have been isolated by DEAE‐cellulose chromatography and characterized by electrophoresis, high pressure liquid chromatography, and a radio‐immunological assay. Six hemoglobin zones have been observed with the following likely compositions. Zone 1: α2ϵ2, or Hb Gower‐2; zone 2: ζ 2ϵ2, or Hb Gower‐1; zone 3: ζ2γ2, or Hb Portland‐I; zone 4: Hb F, or α2γ2; zone 5: a mixture of acetylated Hb Portland‐I and Hb F; zone 6: Hb Bart's, or γ4. The embryonic Hbs (zones 1, 2, and 3) constituted 50%–75% of the total Hb present; the quantities varied from one experiment to the other. Both Hb Gower‐1 and Hb Gower‐2 were present. The γ chain was heterogeneous and contained the Gγ,AγI, and AγT types in a ratio of about 4:2:1, indicating a heterozygosity for the Ile → Thr substitution at position γ75. The methodology used can be applied for additional studies evaluating quantitative changes in Hb types due to in vitro manipulations. | en_GB |
dc.description.sponsorship | This research was supported in part by USPHS research grants HLB-05168, HLB 23736, CA 18185-06, and CA 17533-06 and by designated research funds of the Veterans Administration. This paper is contribution #0651 from the Department of Cell and Molecular Biology of the Medical College of Georgia. | en_GB |
dc.language.iso | en | en_GB |
dc.publisher | Wiley Online Library | en_GB |
dc.rights | info:eu-repo/semantics/restrictedAccess | en_GB |
dc.subject | Leukemia, Experimental | en_GB |
dc.subject | Hemoglobin -- Synthesis | en_GB |
dc.subject | Fetal hemoglobin | en_GB |
dc.title | Identification and quantitation of embryonic and three types of fetal hemoglobin produced on induction of the human pluripotent leukemia cell line K‐562 with hemin | en_GB |
dc.type | article | en_GB |
dc.rights.holder | The copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder. | en_GB |
dc.description.reviewed | peer-reviewed | en_GB |
dc.identifier.doi | 10.1002/ajh.2830120102 | - |
dc.publication.title | American Journal of Hematology | en_GB |
Appears in Collections: | Scholarly Works - FacM&SPB |
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