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dc.identifier.citationBaron, B. (2011). Novel interactions of the GABPα Transcription Factor Complex (Doctoral dissertation).en_GB
dc.description.abstractGA-binding protein α, (GABPα), also referred to NRF-2 or E4TF-1 in humans, is a transcription factor found on Chromosome 21 that forms part of the ETS (E-TwentySix) transcription factor family. It is the only member of this family that forms an obligate heteromeric protein complex due to the lack of a transactivation domain. It forms heterodimers or tetramers with GABPβ and can also recruit other co-factors. This allows GABP to cover a multitude of roles in transcription control. In this study, a search for novel GABPα interactors was carried out using a Y east-Two Hybrid Assay. From the proteins isolated, five were chosen for further analyses: GABP γ, FHL2, NELF-E, TXN and Hypothetical Protein LOC124512 (EBM). Their interaction with GABPα was then tested for physical binding by co-IP and functionally analysed by luciferase assay. Of these five proteins, GABPγ,, FHL2, NELF-E, TXN and EBM showed a functional response but only GABPγ,, FHL2 and EBM were isolated by co-IP. Mechanisms are being proposed for each functional interaction in an attempt to offer a window into possible GABPα implicated pathogenesis. Key words: GABPα, protein interactions, FHL2, NELF-E, TXN, Hypothetical Protein LOC124512en_GB
dc.subjectProteins -- Conformationen_GB
dc.titleNovel interactions of the GABPα transcription factor complexen_GB
dc.rights.holderThe copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holderen_GB
dc.publisher.institutionUniversity of Malta-
dc.publisher.departmentFaculty of Medicine and Surgery. Department of Anatomyen_GB
dc.contributor.supervisorSchembri-Wismayer, Pierre-
dc.contributor.creatorBaron, Byron-
Appears in Collections:Dissertations - FacM&S - 2011
Dissertations - FacM&SAna - 2011

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