MAPK3 deficiency drives autoimmunity via DC arming

Abstract

DC are professional APC that instruct T cells during the inflammatory course of EAE. We have previously shown that MAPK3 (Erk1) is important for the induction of T-cell anergy. Our goal was to determine the influence of MAPK3 on the capacity of DC to arm T-cell responses in autoimmunity. We report that DC from Mapk3/ mice have a significantly higher membrane expression of CD86 and MHC-II and – when loaded with the myelin oligoden- drocyte glycoprotein – show a superior capacity to prime naı ̈ve T cells towards an inflammatory phenotype than Mapk31/1 DC. Nonetheless and as previously described, Mapk3/mice were only slightly but not significantly more susceptible to myelin oligodendrocyte glycoprotein-induced EAE than WT littermate mice. However, Mapk31/1 mice engrafted with Mapk3/BM (KO-WT) developed a severe form of EAE, in direct contrast to WT-KO mice, which were even less sick than control WT-WT mice. An infiltration of DC and accumulation of Th17 cells was also observed in the CNS of KO-WT mice. Therefore, triggering of MAPK3 in the periphery might be a therapeutic option for the treatment of neuroinflammation since absence of this kinase in the immune system leads to severe EAE.