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Title: Placental villous expression of TNF α and IL‐10 and effect of oxygen tension in euploid early pregnancy failure
Authors: Calleja-Agius, Jean
Jauniaux, Eric
Muttukrishna, Shanthi
Keywords: Interleukin-10
Tumor necrosis factor -- Receptors
Issue Date: 2012
Publisher: Wiley-Blackwell Publishing, Inc.
Citation: Calleja-Agius, J., Jauniaux, E., & Muttukrishna, S. (2012). Placental villous expression of TNF α and IL‐10 and effect of oxygen tension in euploid early pregnancy failure. American Journal of Reproductive Immunology, 67(6), 515-525.
Abstract: Problem: The objective was to investigate placental inflammation in chromosomally normal miscarriages in vivo and in vitro. Method of study: Chorionic villous tissue was collected from missed miscarriages and normal gestation-matched controls and cultured at 6 and 20% O2 concentrations. Tissue was karyotyped. Flowcytometric bead arrays and real-time PCR were carried out for protein and gene expression studies. Results: The levels of TNFα and IL-10 were significantly (P < 0.005 and P < 0.05) higher, and the levels of TNF-R1 and TNF-R2 were significantly (P < 0.01 and P < 0.05) lower in culture conditioned medium of villous explants of miscarriages compared to control group. Villous tissue homogenates from miscarriages contained significantly (P < 0.005) lower levels of TNF-R1. There was a significant O2-dependent increase in the secretion of IL-10 (P < 0.01) and decrease in TNFα/IL-10 ratio (P < 0.005) in the culture medium in both groups. Conclusion: Increased levels of TNFα and decreased levels of receptors in miscarriage villous tissue confirm an excessive placental inflammation in miscarriage patients.
Appears in Collections:Scholarly Works - FacM&SAna

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