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dc.contributor.authorSuleiman, Sherif-
dc.contributor.authorDi Fiore, Riccardo-
dc.contributor.authorCassar, Analisse-
dc.contributor.authorFormosa, Melissa Marie-
dc.contributor.authorCalleja-Agius, Jean-
dc.contributor.authorSchembri-Wismayer, Pierre-
dc.identifier.citationSuleiman, S., Di Fiore, R., Cassar, A., Formosa, M. M., Calleja-Agius, J., & Schembri-Wismayer, P. (2020). Anticancer effects of an extract from a local planarian species on human acute myeloid leukemia HL-60 cells in vitro. Biomedicine & Pharmacotherapy, 130, 110549.en_GB
dc.description.abstractCurrent anti-cancer drugs can cause many undesirable side effects to patients. Thus, there is a constant need to develop alternative therapeutic drugs. Bioactive compounds derived from natural products including animals, plants and microorganisms are being actively studied as sources for anticancer treatments. Freshwater planarians are important models for stem cell research and regeneration. However, to date, no studies on the biological activities of planaria extracts on cancer have been published. The aim of this study was to examine the potential antitumoral activity of the extract from planaria species-Malta (PSM) on human acute myeloid leukemia (AML) HL-60 cells. Antiproliferative activity was studied in terms of proliferation, apoptosis and differentiation. The expression of genes involved in the regulation of these important cellular processes was also analyzed using real-time PCR. PSM extract exhibited a selective cytotoxic effect on HL-60 cells when compared to normal lymphocytes. Furthermore, cell cycle analysis and Annexin V/PI assay showed that the extract induced apoptosis in HL-60 cells. The PSM extract induced myeloid differentiation with HL-60 cells showing a decreased nucleo/cytoplasmic ratio, an increase in nitroblue tetrazolium-positive cells, and CD11b- and CD14-positive cells. Finally, we also found that the PSM extract increased the expression of CEBPA, CEBPB, CEBPE, SPI1, BAX, CDKN1A and CDKN2C; whereas it reduced the expression of c-MYC and BCL2. This is the first study to reveal the antiproliferative, cytotoxic, and differentiation potential of PSM on HL-60 cells and suggests that it may have considerable potential for development as a novel natural product-based anticancer agent against AML.en_GB
dc.publisherElsevier Massonen_GB
dc.subjectCell proliferationen_GB
dc.titleAnticancer effects of an extract from a local planarian species on human acute myeloid leukemia HL-60 cells in vitroen_GB
dc.rights.holderThe copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder.en_GB
dc.publication.titleBiomedicine & Pharmacotherapyen_GB
Appears in Collections:Scholarly Works - FacM&SAna

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