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dc.contributor.authorSuleiman, Sherif-
dc.contributor.authorDi Fiore, Riccardo-
dc.contributor.authorCassar, Analisse-
dc.contributor.authorFormosa, Melissa Marie-
dc.contributor.authorSchembri-Wismayer, Pierre-
dc.contributor.authorCalleja-Agius, Jean-
dc.identifier.citationSuleiman, S., Di Fiore, R., Cassar, A., Formosa, M. M., Schembri-Wismayer, P., & Calleja-Agius, J. (2020). Axolotl Ambystoma mexicanum extract induces cell cycle arrest and differentiation in human acute myeloid leukemia HL-60 cells. Tumor Biology, 10.1177/1010428320954735.en_GB
dc.description.abstractAcute myeloid leukemia is the most common form of acute leukemia in adults, constituting about 80% of cases. Although remarkable progress has been made in the therapeutic scenario for patients with acute myeloid leukemia, research and development of new and effective anticancer agents to improve patient outcome and minimize toxicity is needed. In this study, the antitumor activity of axolotl (AXO) Ambystoma mexicanum crude extract was assessed in vitro on the human acute myeloid leukemia HL-60 cell line. The anticancer activity was evaluated in terms of ability to influence proliferative activity, cell viability, cell cycle arrest, and differentiation. Moreover, gene expression analysis was performed to evaluate the genes involved in the regulation of these processes. The AXO crude extract exhibited antiproliferative but not cytotoxic activities on HL-60 cells, with cell cycle arrest in the G0/G1 phase. Furthermore, the AXO-treated HL-60 cells showed an increase in both the percentage of nitroblue tetrazolium positive cells and the expression of CD11b, whereas the proportion of CD14-positive cells did not change, suggesting that extract is able to induce differentiation toward the granulocytic lineage. Finally, the treatment with AXO extract caused upregulation of CEBPA, CEBPB, CEBPE, SPI1, CDKN1A, and CDKN2C, and downregulation of c-MYC. Our data clearly show the potential anticancer activity of Ambystoma mexicanum on HL-60 cells and suggest that it could help develop promising therapeutic agents for the treatment of acute myeloid leukemia.en_GB
dc.publisherSage Publications Ltd.en_GB
dc.subjectAmbystoma mexicanumen_GB
dc.subjectNatural productsen_GB
dc.subjectCell cycleen_GB
dc.subjectCell differentiationen_GB
dc.titleAxolotl Ambystoma mexicanum extract induces cell cycle arrest and differentiation in human acute myeloid leukemia HL-60 cellsen_GB
dc.rights.holderThe copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder.en_GB
dc.publication.titleTumor Biologyen_GB
Appears in Collections:Scholarly Works - FacM&SAna

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