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dc.identifier.citationCaruana, N.A. (2020). Analysis of FeSOD from E. coli (Bachelor's dissertation).en_GB
dc.description.abstractDue to their central position in various physiological conditions, and their involvement in a broad range of diseases, the importance of free radicals in biology has increased dramatically in recent years. Scientists have shown great interest in investigating the roles these radicals perform in illness, and how they counteract the normal physiological environment. In 1954, Gershman first proposed the idea that the toxicity of oxygen is due to its capacity to produce free radicals. Via their study, Commoner et al (1954) confirmed that in the same year free radicals appeared in biological materials. Superoxide dismutase (EC is an enzyme that alternately catalyses dismutation of superoxide to molecular oxygen (O2) and hydrogen peroxide (H2O2) (Sheng et al., 2014). Superoxide is a by-product of oxygen metabolism that causes all kinds of cell damage when left unregulated. The aim of this research was to attempt to analyse the activity of 2 mutant proteins; FeSOD dblDGF and FeSOD MIH to potentially gain further understanding of the key residues that are important for protein selectivity and specificity. The results from testing showed that FeSOD dblDGF elicited 35% activity relative to wild type FeSOD, whilst FeSOD MIH showed almost no activity at all. Given the limited testing, it was impossible to ascertain what is happening structurally or chemically to these proteins, so the activity exhibited by these proteins cannot be fully explained or accounted for, in terms of specificity and selectivity, using this research data alone.en_GB
dc.subjectSuperoxide dismutaseen_GB
dc.subjectEscherichia colien_GB
dc.subjectOxygen -- Metabolismen_GB
dc.titleAnalysis of FeSOD from E. colien_GB
dc.rights.holderThe copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder.en_GB
dc.publisher.institutionUniversity of Maltaen_GB
dc.publisher.departmentFaculty of Medicine and Surgery. Department of Physiology and Biochemistryen_GB
dc.contributor.creatorCaruana, Nicholas Anthony-
Appears in Collections:Dissertations - FacM&S - 2020
Dissertations - FacM&SPB - 2020

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