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dc.contributor.authorTeixeira, Sofia Rodrigues-
dc.contributor.authorLloyd, Catherine-
dc.contributor.authorYao, Seydou-
dc.contributor.authorGazze, Andrea Salvatore-
dc.contributor.authorWhitaker, Iain S.-
dc.contributor.authorFrancis, Lewis W.-
dc.contributor.authorConlan, Robert Steven-
dc.contributor.authorAzzopardi, Ernest A.-
dc.identifier.citationTeixeira, S. R., Lloyd, C., Yao, S., Gazze, A. S., Whitaker, I. S., Francis, L.,...Azzopardi, E. (2016). Polyaniline-graphene based α-amylase biosensor with a linear dynamic range in excess of 6 orders of magnitude. Biosensors and Bioelectronics, 85, 395-402.en_GB
dc.description.abstractα-amylase is an established marker for diagnosis of pancreatic and salivary disease, and recent research has seen a substantial expansion of its use in therapeutic and diagnostic applications for infection, cancer and wound healing. The lack of bedside monitoring devices for α-amylase detection has hitherto re- stricted the clinical progress of such applications. We have developed a highly sensitive α-amylase immunosensor platform, produced via in situ electropolymerization of aniline onto a screen-printed graphene support (SPE). Covalently binding an α- amylase specific antibody to a polyaniline (PANI) layer and controlling device assembly using electro- chemical impedance spectroscopy (EIS), we have achieved a highly linear response against α-amylase concentration. Each stage of the assembly was characterized using a suite of high-resolution topo- graphical, chemical and mechanical techniques. Quantitative, highly sensitive detection was demon- strated using an artificially spiked human blood plasma samples. The device has a remarkably wide limit of quantification (0.025–1000 IU/L) compared to α-amylase assays in current clinical use. With potential for simple scale up to volume manufacturing though standard semiconductor production techniques and subsequently clinical application, this biosensor will enable clinical benefit through early disease de- tection, and better informed administration of correct therapeutic dose of drugs used to treat α-amylase related diseases.en_GB
dc.description.sponsorshipThis work is funded by the European Social Fund (ESF80300) through the European Union's Convergence programme adminis- tered by the Welsh Government, Knowledge Economy Skills Scholarships (KESS), and St David's Medical Foundation.en_GB
dc.subjectPancreas -- Diseases -- Diagnosisen_GB
dc.subjectBiosensor industryen_GB
dc.titlePolyaniline-graphene based α-amylase biosensor with a linear dynamic range in excess of 6 orders of magnitudeen_GB
dc.rights.holderThe copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder.en_GB
dc.publication.titleBiosensors and Bioelectronicsen_GB
Appears in Collections:Scholarly Works - FacM&SAna

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