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DC Field | Value | Language |
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dc.contributor.author | Durcan, Niamh | |
dc.contributor.author | Costello, Richard W. | |
dc.contributor.author | McLean, Graham W. | |
dc.contributor.author | Blusztajn, Jan | |
dc.contributor.author | Madziar, Beata | |
dc.contributor.author | Fenech, Anthony G. | |
dc.contributor.author | Hall, Ian P. | |
dc.contributor.author | Gleich, Gerard J. | |
dc.contributor.author | McGarvey, Lorcan | |
dc.contributor.author | Walsh, Marie-Therese | |
dc.date.accessioned | 2016-03-21T15:35:03Z | |
dc.date.available | 2016-03-21T15:35:03Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | American Journal of Respiratory Cell and Molecular Biology. 2006, Vol.34(6), p. 775–786. | en_GB |
dc.identifier.uri | https://www.um.edu.mt/library/oar//handle/123456789/9056 | |
dc.description.abstract | Eosinophils are observed to localize to cholinergic nerves in a variety of inflammatory conditions such as asthma, rhinitis, eosinophilic gastroenteritis, and inflammatory bowel disease, where they are also responsible for the induction of cell signaling.Wehypothesized that a consequence of eosinophil localization to cholinergic nerves would involve a neural remodeling process. Eosinophil co-culture with cholinergic IMR32 cells led to increased expression of the M2 muscarinic receptor, with this induction being mediated via an adhesion-dependent release of eosinophil proteins, including major basic protein and nerve growth factor. Studies on the promoter sequence of the M2 receptor indicated that this induction was initiated at a transcription start site 145 kb upstream of the gene-coding region. This promoter site contains binding sites for a variety of transcription factors including SP1, AP1, and AP2. Eosinophils also induced the expression of several cholinergic genes involved in the synthesis, storage, and metabolism of acetylcholine, including the enzymes choline acetyltransferase, vesicular acetylcholine transferase, and acetylcholinesterase. The observed eosinophil-induced changes in enzyme content were associated with a reduction in intracellular neural acetylcholine but an increase in choline content, suggesting increased acetylcholine turnover and a reduction in acetylcholinesterase activity, in turn suggesting reduced catabolism of acetylcholine. Together these data suggest that eosinophil localization to cholinergic nerves induces neural remodeling, promoting a cholinergic phenotype. | en_GB |
dc.language.iso | en | en_GB |
dc.publisher | The American Thoracic Society | en_GB |
dc.rights | info:eu-repo/semantics/openAccess | en_GB |
dc.subject | Eosinophils | en_GB |
dc.subject | Acetylcholine -- Analysis | en_GB |
dc.subject | Eosinophilia | en_GB |
dc.title | Eosinophil-Mediated Cholinergic Nerve Remodeling | en_GB |
dc.type | article | en_GB |
dc.rights.holder | The copyright of this work belongs to the author(s)/publisher. The rights of this work are as defined by the appropriate Copyright Legislation or as modified by any successive legislation. Users may access this work and can make use of the information contained in accordance with the Copyright Legislation provided that the author must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the prior permission of the copyright holder. | en_GB |
dc.description.reviewed | peer-reviewed | en_GB |
dc.identifier.doi | 10.1165/rcmb.2005-0196OC | |
Appears in Collections: | Scholarly Works - FacM&SCPT |
Files in This Item:
File | Description | Size | Format | |
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Eosinophil-Mediated Cholinergic Nerve Remodeling.pdf | 410.48 kB | Adobe PDF | View/Open |
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