| CODE | MMB3003 | ||||||||||||
| TITLE | Methods of Biomedical Analysis | ||||||||||||
| UM LEVEL | 03 - Years 2, 3, 4 in Modular Undergraduate Course | ||||||||||||
| MQF LEVEL | 6 | ||||||||||||
| ECTS CREDITS | 8 | ||||||||||||
| DEPARTMENT | Centre for Molecular Medicine and Biobanking | ||||||||||||
| DESCRIPTION | This study-unit is sub-divided into two sections with the first part being theoretical and the second being applied. The theoretical part consists of a set of lectures aimed at providing students with the scientific principles of major laboratory experimental techniques. The lectures will provide an in-depth understanding of how the selected laboratory techniques can be applied in different scenarios, the basic procedure and the most common modifications used, what kind of samples need to be collected and how these samples should be prepared. The second part comprises lab-based demonstrations, which will provide a run-through of each technique and expand on the specific steps in the procedure, allowing students to get acquainted with the equipment, chemicals, protocols, timings, etc involved in setting up and running of experiments. Study-unit Aims: This study-unit aims to teach students: - about the chemical principles of selected laboratory techniques; - how to adapt a standard protocol to address a specific research question, based on the knowledge of the aforementioned principles; - to acquire confidence and basic ability to perform the selected techniques under supervision. Learning Outcomes: 1. Knowledge & Understanding: By the end of the study-unit the student will be able to: - describe the chemical principles of selected laboratory techniques; - apply their knowledge to implement common practice modifications to such laboratory techniques; - explain the laboratory conditions and how to use basic laboratory equipment; and - apply the practiced techniques to their research work. 2. Skills: By the end of the study-unit the student will be able to: - perform basic troubleshooting using fundamental biochemical reactions; - work confidently and follow standard operating procedure when working in a laboratory setting; - set-up basic laboratory equipment; - operate basic laboratory equipment; - perform daily maintenance of basic laboratory equipment; and - apply appropriate experimental techniques to a specific project design Main Text/s and any supplementary readings: Main Text Students will be provided will all relevant lab technique-related literature - Weissman, S. (1999). CDNA preparation and characterization (Methods in enzymology 303). Academic Press. - Pardee, A., & McClelland, M. (1999). Expression genetics: Differential display (BioTechniques update series). BioTechniques Books. - Burgess, R., & Deutscher, M. (2009). Guide to protein purification(2nd ed., Methods in enzymology 463). Academic Press. - Corthell, J. (2014). Basic molecular protocols in neuroscience : Tips, tricks, and pitfalls. https://www-sciencedirect-com.ejournals.um.edu.mt/science/book/9780128014615 Reference - Walters, N., Estridge, B., & Reynolds, A. (1990). Basic medical laboratory techniques (2nd ed.). Delmar. - Schena, M. (2003). Microarray analysis. Wiley-Liss. |
||||||||||||
| STUDY-UNIT TYPE | Lecture, Independent Study & Practicum | ||||||||||||
| METHOD OF ASSESSMENT |
|
||||||||||||
| LECTURER/S | Byron Baron |
||||||||||||
|
The University makes every effort to ensure that the published Courses Plans, Programmes of Study and Study-Unit information are complete and up-to-date at the time of publication. The University reserves the right to make changes in case errors are detected after publication.
The availability of optional units may be subject to timetabling constraints. Units not attracting a sufficient number of registrations may be withdrawn without notice. It should be noted that all the information in the description above applies to study-units available during the academic year 2023/4. It may be subject to change in subsequent years. |
|||||||||||||