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Title: | Ecballium elaterium: an in vitro source of cucurbitacins |
Authors: | Attard, Everaldo Scicluna-Spiteri, Anthony |
Keywords: | Cucurbitales Cell culture Cucurbitaceae |
Issue Date: | 2001-01-31 |
Publisher: | Elsevier |
Citation: | Attard, E., & Scicluna-Spiteri, A. (2001). Ecballium elaterium: an in vitro source of cucurbitacins. Fitoterapia, 72(1), 46-53. |
Abstract: | Biomass and secondary metabolite accumulation were assayed on a wide range of auxins, cytokinins and their combinations using Ecballium elaterium callus tissue. The best combination was that consisting of α-naphthalene acetic acid (NAA) and benzylamino purine (BAP) (1 mg/l of medium, each). NAA and BAP were set up in a grid to determine concentrations that produced the best callus as regards fresh and dry weights and yield of cucurbitacins, particularly cucurbitacin E. The best combination for callus proliferation consisted of 5 mg/l NAA and 5 mg/l BAP, reaching a peak at week 5. For cucurbitacin production, an optimum yield was obtained at week 3 with 5 mg/l NAA. |
URI: | https://www.um.edu.mt/library/oar//handle/123456789/19460 |
Appears in Collections: | Scholarly Works - InsESRSF |
Files in This Item:
File | Description | Size | Format | |
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fitoartc.pdf Restricted Access | Main article | 203.67 kB | Adobe PDF | View/Open Request a copy |
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