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Title: Oleuropein prevents neuronal death, mitigates mitochondrial superoxide production and modulates autophagy in a dopaminergic cellular model
Authors: Arel-Dubeau, Anne-Marie
Renaud, Justine
Legrand, Manon
Attard, Everaldo
Germain, Marc
Martinoli, Maria-Grazia
Achour, Imene
Keywords: Parkinson’s disease
Neuroprotective agents
Nervous system -- Degeneration
Oxidative stress
Issue Date: 2016-09-08
Publisher: Multidisciplinary Digital Publishing Institute
Citation: Achour, I., Arel-Dubeau, A. M., Renaud, J., Legrand, M., Attard, E., Germain, M., & Martinoli, M. G. (2016). Oleuropein prevents neuronal death, mitigates mitochondrial superoxide production and modulates autophagy in a dopaminergic cellular model. International Journal of Molecular Sciences, 17(8), 1293.
Abstract: Parkinson’s disease (PD) is a progressive neurodegenerative disorder, primarily affecting dopaminergic neurons in the substantia nigra. There is currently no cure for PD and present medications aim to alleviate clinical symptoms, thus prevention remains the ideal strategy to reduce the prevalence of this disease. The goal of this study was to investigate whether oleuropein (OLE), the major phenolic compound in olive derivatives, may prevent neuronal degeneration in a cellular dopaminergic model of PD, differentiated PC12 cells exposed to the potent parkinsonian toxin 6-hydroxydopamine (6-OHDA).We also investigated OLE’s ability to mitigate mitochondrial oxidative stress and modulate the autophagic flux. Our results obtained by measuring cytotoxicity and apoptotic events demonstrate that OLE significantly decreases neuronal death. OLE could also reduce mitochondrial production of reactive oxygen species resulting from blocking superoxide dismutase activity. Moreover, quantification of autophagic and acidic vesicles in the cytoplasm alongside expression of specific autophagic markers uncovered a regulatory role for OLE against autophagic flux impairment induced by bafilomycin A1. Altogether, our results define OLE as a neuroprotective, anti-oxidative and autophagy-regulating molecule, in a neuronal dopaminergic cellular model.
Description: This work was funded by a Natural Sciences and Engineering Research Council of Canada (NSERC) grant to Maria-Grazia Martinoli.
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