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|Title:||Biocompatibility of two commercial forms of mineral trioxide aggregate|
Montesin, Franco E.
Pitt Ford, Thomas R.
Scanning electron microscopy
|Citation:||Camilleri, J., Montesin, F. E., Papaioannou, S., McDonald, F., & Pitt Ford, T. R. (2004). Biocompatibility of two commercial forms of mineral trioxide aggregate. International Endodontic Journal, 37(10), 699-704.|
|Abstract:||Aim: To examine the biocompatibility of two commercial forms of mineral trioxide aggregate (MTA), by evaluating the morphology of an established cell line. Methodology: The two cements were cast on glass cover slips and cured for 1 or 28 days. Saos-2 osteosarcoma cells were trypsinized and seeded at a density of 1 × 105 cells and were then placed in medium over the material-coated coverslips for 1, 5 and 7 days. After these time intervals the media were discarded and the cells fixed. Cell morphological investigation was performed by scanning electron microscopy at various magnifications ranging from x 250 to x 500. The biocompatibility of cement constituents, alusilicate flux and bismuth oxide was also investigated. Results: All cement samples cured for 1 day showed a confluent cell monolayer after 5 and 7 days. The response to both materials was similar. Materials cured for 28 days showed incomplete cell confluence after 1 and 5 days. Alusilicate flux and bismuth oxide did not demonstrate biocompatibility. Conclusions: The 1-day cured samples of two commercial forms of MTA showed good biocompatibility. However, the 28-day cured samples were less biocompatible after 1 and 5 days.|
|Appears in Collections:||Scholarly Works - FacDenRS|
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