Please use this identifier to cite or link to this item: https://www.um.edu.mt/library/oar/handle/123456789/78218
Title: The valorisation of the indigenous grapevine variety, Gellewza, through tissue culture
Authors: Bonello, Mary Anne (2016)
Keywords: Plant metabolites -- Malta
Grapes -- Malta
Plant tissue culture -- Malta
Issue Date: 2016
Citation: Bonello, M. A. (2016). The valorisation of the indigenous grapevine variety, Gellewza, through tissue culture (Master's dissertation).
Abstract: The production of secondary metabolites in tissue culture has been considered as an alternative to the cultivation and harvesting of crops intended for this purpose. The present study was aimed at the production of callus derived from a Maltese indigenous variety, Gellewza, and then inoculated onto plant growth regulators enriched Murashige Skoog (MS) media to determine whether metabolites are produced in vitro as well as to determine the best combination of plant growth regulators needed for the production of these metabolites. The Gellewza material was obtained from certified and potential vineyards. Cuttings were tested for common grapevine viruses, forced to produce shoots and following surface sterilisation, explants were allowed to acclimatise on MS medium. Callus production was induced at this stage, and a Gellewza strain with a high callus yield was selected for further studies. Callus was weighed and placed in different combinations of plant growth regulators. The resultant cultures were allowed to develop and the final callus was assessed for the presence of metabolites. From results obtained, it was observed that the best callus production was obtained by auxin enriched MS media. In fact indole acetic acid and indole acetic acid /6-benzylaminopurine enhanced biomass accumulation (3.036g and 3.391 g) as opposed to the others (<1.973g). On the other hand parameters showing the presence of flavonoids (tonality), particularly anthocyanins and total polyphenols, were optimum in the presence of cytokinins particularly 6-benzylaminopurine (Tonality (3.799), anthocyanins (24.1 mg/kg), total polyphenolic content (0.142%). This is the first study to describe the production of polyphenols in Gellewza callus cultures. The findings may stimulate further research along this direction.
Description: M.SC.RURAL SCI.
URI: https://www.um.edu.mt/library/oar/handle/123456789/78218
Appears in Collections:Dissertations - InsES - 2016
Dissertations - InsESRSF - 2016

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