Please use this identifier to cite or link to this item: https://www.um.edu.mt/library/oar/handle/123456789/98128
Title: Active hexose-correlated compound down-regulates sex-determining region Y-box 2 of pancreatic cancer cells
Authors: Nawata, Junya
Kuramitsu, Yasuhiro
Wang, Yufeng
Kitagawa, Takao
Tokuda, Kazuhiro
Baron, Byron
Akada, Junko
Suenaga, Shigeyuki
Kaino, Seiji
Maehara, Shin-Ichiro
Maehara, Yoshihiko
Sakaida, Isao
Nakamura, Kazuyuki
Keywords: Genetic transcription
Embryonic stem cells -- Research
Biological response modifiers
Cancer -- Alternative treatment
Mushrooms -- Therapeutic use
Glucans -- Therapeutic use
Pancreas -- Cancer
Cancer -- Chemotherapy
Proteins
Issue Date: 2014
Publisher: International Institute of Anticancer Research
Citation: Nawata, J., Kuramitsu, Y., Wang, Y., Kitagawa, T., Tokuda, K., Baron, B., ... & Nakamura, K. (2014). Active hexose-correlated compound down-regulates sex-determining region Y-box 2 of pancreatic cancer cells. Anticancer Research, 34(9), 4807-4811.
Abstract: Background/Aim: Active hexose-correlated compound (AHCC) is an extract of basidiomycete mushroom. It has been used as health food due to its efficacy of enhancing antitumor effects and reducing adverse effects of chemotherapy. Our previous research showed that AHCC down-regulated heat-shock protein (HSP)-27 and exhibited cytotoxic effects against gemcitabine-resistant pancreatic cancer cells. Sex-determining region Y-box 2 (SOX2) is reported to be up-regulated in other kinds of cancer cells and involved in carcinogenesis and malignancy. The aim of this study was to investigate the effects of AHCC on protein expression of SOX2 in the gemcitabine-resistant pancreatic cancer cell line KLM1-R.
Materials and Methods: AHCC was applied to KLM1-R cells and expression of SOX2 was analyzed by western blotting.
Results: AHCC down-regulated SOX2 in KLM1-R cells. Nanog and Oct4, co-workers of SOX2 in maintaining pluripotency, did not exhibit any significant change in protein expression.
Conclusion: We showed the potential of AHCC to be a candidate for combinatorial therapy in anticancer drug regimens. This result suggests that the target of AHCC in expressing therapeutic efficacy was not the pluripotent cells such as cancer stem cells (CSCs) but SOX2-specific.
URI: https://www.um.edu.mt/library/oar/handle/123456789/98128
Appears in Collections:Scholarly Works - CenMMB

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