CUB domain-containing protein 1 (CDCP1) is down-regulated by active hexose-correlated compound in human pancreatic cancer cells

Abstract

Background/Aim: We have previously reported that treatment of pancreatic cancer cells with active hexosecorrelated compound (AHCC), an extract of a basidiomycete mushroom, decreases the levels of tumor-associated proteins including heat-shock protein 27 (HSP27), heat shock factor 1 (HSF1) and sex-determining region Y-box 2 (SOX2). The transmembrane glycoprotein, CUB domain-containing protein 1 (CDCP1) has been reported to be up-regulated in various cancers, and be associated with invasion and metastasis. The aim of this study was to examine the effect of AHCC on the expression of CDCP1 in KLM1-R cells. Materials and Methods: Gemcitabine-resistant pancreatic cancer cells (KLM1-R) were treated with AHCC (10 mg/ml) for 48 h. Western blot analysis of cell extracts with anti-CDCP1 or antiactin antibodies was performed to assess the expression of CDCP1. Results: Expression of CDCP1 was reduced by AHCC treatment of KLM1-R cells, whereas expression of actin was not affected. The ratio of intensities of CDCP1/actin in AHCCtreated KLM1-R cells was significantly suppressed (p<0.05) compared to untreated cells. Conclusion: AHCC downregulated CDCP1 expression and inhibited the malignant progression of pancreatic cancer cells.