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https://www.um.edu.mt/library/oar/handle/123456789/98611
Title: | AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS - FLI1 transcriptional activity in Ewing’s sarcoma cells |
Authors: | Kitagawa, Takao Kobayashi, Daiki Baron, Byron Okita, Hajime Miyamoto, Tatsuo Takai, Rie Paudel, Durga Ohta, Tohru Asaoka, Yoichi Tokunaga, Masayuki Nakagawa, Koji Furutani-Seiki, Makoto Araki, Norie Kuramitsu, Yasuhiro Kobayashi, Masanobu |
Keywords: | Tumors in children Ewing's sarcoma Cancer in children Transcription factors |
Issue Date: | 2022 |
Publisher: | bioRxiv |
Citation: | Kitagawa, T., Kobayashi, D., Baron, B., Okita, H., Miyamoto, T., Takai, R., ... & Kobayashi, M. (2022). AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing’s sarcoma cells. bioRxiv. |
Abstract: | Ewing’s sarcoma is the second most common bone malignancy in children or young adults and is caused by an oncogenic transcription factor by a chromosomal translocation between the EWSR1 gene and the ETS transcription factor family. However, the transcriptional mechanism of EWS-ETS fusion proteins is still unclear. To identify the transcriptional complexes of EWS-ETS fusion transcription factors, we applied a proximal labeling system called BioID in Ewing’s sarcoma cells. We identified AHDC1 as a proximal protein of EWS-ETS fusion proteins. AHDC1 knockdown showed a reduced cell growth and transcriptional activity of EWS-FLI1. AHDC1 knockdown also reduced BRD4 and BRG1 protein levels, both known as interacting proteins of EWS-FLI1. In addition, AHDC1 co-localized with BRD4. Our results suggest that AHDC1 supports cell growth through EWS-FLI1. |
URI: | https://www.um.edu.mt/library/oar/handle/123456789/98611 |
Appears in Collections: | Scholarly Works - CenMMB |
Files in This Item:
File | Description | Size | Format | |
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AT-hook_DNA-binding_motif-containing_protein_one_knockdown_downregulates_EWS-FLI1_transcriptional_activity_in_Ewing’s_sarcoma_cells(2022).pdf | 1.75 MB | Adobe PDF | View/Open |
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