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Title: | Cortistatin-14 mediates its anticonvulsant effects Via sst2 and sst3 but not ghrelin receptors |
Authors: | Aourz, Najat Portelli, Jeanelle Coppens, Jessica Bundel, Dimitri De Di Giovanni, Giuseppe Van Eeckhaut, Ann Michotte, Yvette Smolders, Ilse J. |
Keywords: | Epilepsy Pilocarpine Somatostatin Receptors, Ghrelin |
Issue Date: | 2014 |
Publisher: | Wiley-Blackwell Publishing Ltd. |
Citation: | Aourz, N., Portelli, J., Coppens, J., De Bundel, D., Di Giovanni, G., Van Eeckhaut, A., Michotte, Y., & Smolders, I. (2014). Cortistatin-14 mediates its anticonvulsant effects Via sst2 and sst3 but not ghrelin receptors. CNS Neuroscience & Therapeutics, 20(7), 662-670. |
Abstract: | Cortistatin (CST)-14, a neuropeptide that is structurally and functionally related to somatostatin-14 (SRIF) binds all five somatostatin receptor subtypes (sst1–sst5). Using in vivo microdialysis and telemetry-based electroencephalographic recordings, we provide the first experimental evidence for anticonvulsive effects of CST-14 in a pilocarpine-induced seizure model in rats and mice and for the involvement of sst2 and sst3 receptors in these anticonvulsant actions of CST-14. Both receptor subtypes are required for the anticonvulsant effects of CST-14 given that co-perfusion of a selective sst2 antagonist (cyanamid15486) or a selective sst3 antagonist (SST3-ODN-8) reversed anticonvulsant effect of CST-14, and this, independently of each other. Next, as the ghrelin receptor has been proposed as a target for the biological effects of CST-14, we used ghrelin receptor knockout mice and their wild type littermates to study the involvement of this receptor in the anticonvulsive actions of CST-14. Our results show a significant decrease in seizure duration in both genotypes when CST-14 treated mice were compared with corresponding control animals receiving only pilocarpine. In addition, this CST-14-induced decrease was comparable in both genotypes. We here thus provide the first evidence that ghrelin receptors are not involved in mediating anticonvulsant actions of CST-14 in vivo. |
URI: | https://www.um.edu.mt/library/oar//handle/123456789/22410 |
Appears in Collections: | Scholarly Works - FacM&SPB |
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Aourz et al CNS N&T.pdf Restricted Access | 654.22 kB | Adobe PDF | View/Open Request a copy |
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