Please use this identifier to cite or link to this item: https://www.um.edu.mt/library/oar/handle/123456789/22763
Title: Selective activation of 5-HT2C receptors stimulates GABA-ergic function in the rat substantia nigra pars reticulate : a combined in vivo electrophysiological and neurochemical study
Authors: Invernizzi, Roberto William
Pierucci, Massimo
Calcagno, E.
Di Giovanni, Giuseppe
Di Matteo, Vincenzo
Benigno, Arcangelo
Esposito, Ennio
Keywords: Substantia nigra
Basal ganglia
GABAergic neurons
Receptor, Serotonin, 5-HT2C
Electrophysiology
Microdialysis
Issue Date: 2007
Publisher: Pergamon Press
Citation: Invernizzia, R. W., Pierucci, M., Calcagno, E., Di Giovanni, G., Di Matteo, V., Benignoc, A., & Esposito, E. (2007). Selective activation of 5-HT2C receptors stimulates GABA-ergic function in the rat substantia nigra pars reticulate : a combined in vivo electrophysiological and neurochemical study. Neuroscience, 144(4), 1523-1535.
Abstract: In vivo electrophysiology and microdialysis were used to investigate the physiological role of 5-HT2C receptors in the control of substantia nigra pars reticulata (SNr) function. Extracellular single-unit recordings were performed from putative GABA-containing neurons in the SNr of anesthetized rats, and local GABA release was studied by in vivo microdialysis in the SNr of awake freely-moving rats. Systemic administration of the selective 5-HT2C receptor agonist (S)-2-(chloro-5-fluoro-indol-1-yl)-1-methylethylamine 1:1 C4H4O4 (RO 60-0175) caused a dose-dependent excitation of about 30% of the SNr neurons recorded. However, the remaining neurons were either inhibited or unaffected by systemic RO 60-0175, in similar proportion. Local application of RO 60-0175 by microiontophoresis caused excitation in the majority of SNr neurons tested (48%), whereas a group of neurons was inhibited (16%) or unaffected (36%). Both the excitatory and the inhibitory effects of systemic and microiontophoretic RO 60-0175 were completely prevented by pretreatment with SB 243213 [5-methyl-1-({2-[(2-methyl-3-pyridyl)oxy]-5-pyridyl}carbamoyl)-6-trifluoromethylindoline], a selective and potent 5-HT2C receptor antagonist. Consistent with these electrophysiological data, both systemic and intranigral administration of RO 60-0175 and m-chlorophenylpiperazine (mCPP), a non-selective 5-HT2C agonist, markedly increased extracellular GABA levels in the SNr. The stimulatory effect of systemic and local RO 60-0175 on GABA release was completely prevented by systemic administration of SB 243213, whereas local application of SB 243213 into the SNr only partially blocked RO 60-0175-induced GABA release. It is concluded that selective activation of 5-HT2C receptors stimulates GABA-ergic function in the SNr, and the clinical relevance of these data is discussed.
URI: https://www.um.edu.mt/library/oar//handle/123456789/22763
Appears in Collections:Scholarly Works - FacM&SPB

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