Core-shell particle column performance in acetylsalicylic acid related substances testing by rapid resolution liquid chromatography

Abstract

Until recently, HPLC column performance was improved by shortening the column and decreasing the particle size below the 2-μm limit. A significant disadvantage of this technique is ultra high back pressures. Recently developed Kinetex® core-shell columns have been designed to achieve sub-2-μm efficiency without the ultra high back pressures by diffusing the mobile phase through the small volume of the stationary phase, the shell. The objective of this study was to investigate the suitability of core-shell column technology for the related substances testing of acetylsalicylic acid using rapid resolution liquid chromatography (RRLC).