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Title: Stimulation of yeast 3-phosphoglycerate kinase gene promoter by paraquat
Authors: Vassallo, Neville
Galea Agius, Dorita
Bannister, William H.
Balzan, Rena
Keywords: Saccharomyces cerevisiae
Oxidative stress
Phosphoglycerate kinase
Issue Date: 2000
Publisher: Elsevier Inc.
Citation: Vassallo, N., Galea, D. R., Bannister, W. H., & Balzan, R. (2000). Stimulation of yeast 3-phosphoglycerate kinase gene promoter by paraquat. Biochemical and Biophysical Research Communications, 270(3), 1036-1040.
Abstract: Yeast cells exposed to adverse conditions employ a number of defense mechanisms in order to respond effectively to the stress and sustain a high proliferation rate. It has been shown that several glycolytic enzymes are induced upon heat treatment of yeast. In this work, we used a reporter plasmid construct to study the effects of oxidative stress, induced by the O•−2-generating compound paraquat (PQ), on the yeast 3-phosphoglycerate kinase gene (PGK) promoter. Our results show that (i) moderate, as opposed to excessive, doses of PQ induce increased stimulation of the PGK promoter, at midlogarithmic phase of growth; and (ii) the thiol antioxidant N-acetylcysteine cancels this stimulatory effect. These observations may represent one aspect of a more general role for glycolysis in maintaining the energy pools of yeast cells under stress.
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