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Title: Preferential modulation of the lateral habenula activity by serotonin-2A rather than -2C receptors : electrophysiological and neuroanatomical evidence
Authors: Delicata, Francis
Bombardi, Cristiano
Pierucci, Massimo
Maio, Roberto di
Deurwaerdere de, Philippe
Di Giovanni, Giuseppe
Keywords: Immunohistochemistry
Brain -- Localization of functions
Brain -- Diseases -- Diagnosis
Affective disorders -- Diagnosis
Neurons -- Physiology
Issue Date: 2018
Publisher: Blackwell Publishing Ltd.
Citation: Delicata, F., Bombardi, C., Pierucci, M., Di Maio, R., De Deurwaerdère, P., & Di Giovanni, G. (2018). Preferential modulation of the lateral habenula activity by serotonin-2A rather than -2C receptors: electrophysiological and neuroanatomical evidence. CNS Neuroscience and Therapeutics, 1–13.
Abstract: Aims: Serotonergic (5-HT) modulation of the lateral habenula (LHb) activity is central in normal and pathologic conditions such as mood disorders. Among the multiple 5-HT receptors (5-HTRs) involved, the 5-HT2CR seems to play a pivotal role. Yet, the role of 5-HT2ARs in the control of the LHb neuronal activity is completely unknown. Methods: Single-cell extracellular recording of the LHb neurons was used in rats to study the effect of the general activation and blockade of the 5-HT2CR and 5-HT2AR with Ro 60-0175 and SB242084, TCB-2 and MDL11939, respectively. The expression of both receptors in the LHb was confirmed using immunohistochemistry. Results: Cumulative doses (5-640 μg/kg, iv) of Ro 60-0175 and TCB-2 affected the activity of 34% and 63% of the LHb recorded neurons, respectively. LHb neurons were either inhibited at low doses or excited at higher doses of the 5-HT2A/CR agonists. SB242084 or MDL11939 (both at 200 μg/kg, iv) did not modify neuronal firing when injected alone, but reverted the bidirectional effects of Ro 60-0175 or TCB-2, respectively. 5-HT2CRs and 5-HT2ARs are expressed in less than the 20% of the LHb neurons, and they neither colocalize nor make heterodimers. Strikingly, only 5-HT2ARs are expressed by the majority of LHb astrocyte cells. Conclusions: Peripheral administration of 5-HT2AR agonist promotes a heterogeneous pattern of neuronal responses in the LHb, and these effects are more prominent than those induced by the 5-HT2CR activation.
Description: The University of Malta Research Fund; the MCST, Grant/Award Number: R&I-2013-014.
Appears in Collections:Scholarly Works - FacM&SPB

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