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Title: Novel interactions of the GABPα transcription factor complex
Authors: Baron, Byron
Keywords: Genes
Proteins -- Conformation
Issue Date: 2011
Citation: Baron, B. (2011). Novel interactions of the GABPα Transcription Factor Complex (Doctoral dissertation).
Abstract: GA-binding protein α, (GABPα), also referred to NRF-2 or E4TF-1 in humans, is a transcription factor found on Chromosome 21 that forms part of the ETS (E-TwentySix) transcription factor family. It is the only member of this family that forms an obligate heteromeric protein complex due to the lack of a transactivation domain. It forms heterodimers or tetramers with GABPβ and can also recruit other co-factors. This allows GABP to cover a multitude of roles in transcription control. In this study, a search for novel GABPα interactors was carried out using a Y east-Two Hybrid Assay. From the proteins isolated, five were chosen for further analyses: GABP γ, FHL2, NELF-E, TXN and Hypothetical Protein LOC124512 (EBM). Their interaction with GABPα was then tested for physical binding by co-IP and functionally analysed by luciferase assay. Of these five proteins, GABPγ,, FHL2, NELF-E, TXN and EBM showed a functional response but only GABPγ,, FHL2 and EBM were isolated by co-IP. Mechanisms are being proposed for each functional interaction in an attempt to offer a window into possible GABPα implicated pathogenesis. Key words: GABPα, protein interactions, FHL2, NELF-E, TXN, Hypothetical Protein LOC124512
Description: PH.D.
Appears in Collections:Dissertations - FacM&S - 2011
Dissertations - FacM&SAna - 2011

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