A simple HPLC-UV method for the determination of clindamycin in human plasma

Abstract

This study describes a simple high performance liquid chromatographic (HPLC) method for the determination of clindamycin in plasma. Analysis was carried out using a Varian® Pro Star HPLC unit equipped with an online degasser. A reversed-phase ACE® C18 column of dimensions 250x4.6mm, particle size 5μm was used. The mobile phase was made up of 0.02M disodiumhydrogen phosphate buffer (pH of 2.9) and acetonitrile at a ratio of 71:29 v/v, running through the column at a flow rate of 1.5ml/min and with ultraviolet (UV) detection set at a wavelength of 195nm. Clindamycin was separated from plasma proteins by protein precipitation with ice cold acetonitrile. Clindamycin and the internal standard phenobarbitone eluted after 3.96 and 7 minutes respectively. The method was validated for linearity in the working concentration range of 0.5-20μg/ml. Linearity was observed with a coefficient of determination (r2) of 0.990. The recoveries obtained were all above 82% and the limit of quantification and limit of detection were 0.2μg/ml and 0.1μg/ml respectively.