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https://www.um.edu.mt/library/oar/handle/123456789/48694| Title: | A simple HPLC–UV method for the determination of ciprofloxacin in human plasma |
| Authors: | Vella Szijj, Janis Busuttil, Francesca Sammut Bartolo, Nicolette Sammut, Carmel Ferrito, Victor Serracino-Inglott, Anthony Azzopardi, Lilian M. LaFerla, Godfrey |
| Keywords: | High performance liquid chromatography Ciprofloxacin Protein products industry Plasma chemistry |
| Issue Date: | 2015 |
| Publisher: | Elsevier |
| Citation: | Vella, J., Busuttil, F., Bartolo, N. S., Sammut, C., Ferrito, V., Serracino-Inglott, A., ... & LaFerla, G. (2015). A simple HPLC–UV method for the determination of ciprofloxacin in human plasma. Journal of Chromatography B, 989, 80-85. |
| Abstract: | A rapid and sensitive HPLC–UV method for the determination of ciprofloxacin in human plasma is described. Protein precipitation with acetonitrile was used to separate the drug from plasma protein. An ACE® 5 C18 column (250 mm × 4.6 mm, 5 m) with an isocratic mobile phase consisting of phosphate buffer (pH 2.7) and acetonitrile (77:23, v/v) was used for separation. The UV detector was set at 277 nm. The method was validated in the linear range of 0.05–8 g/ml with acceptable inter- and intra-assay precision, accuracy and stability. The method is simple and rapid and can be used to quantify this widely used antibiotic in the plasma of patients suffering from Peripheral Arterial Disease. |
| URI: | https://www.um.edu.mt/library/oar/handle/123456789/48694 |
| Appears in Collections: | Scholarly Works - FacM&SPha |
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|---|---|---|---|---|
| A_simple_HPLCUV_method_for_the_determination_of_ciprofloxacin_in_human_plasma.pdf Restricted Access | 602.34 kB | Adobe PDF | View/Open Request a copy |
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