The use of platelet additive solution for the preparation of pooled platelets

Abstract

By the time my study was validated by the quality assurance manager and it began to be used as a routine procedure, there were major problems especially during the 5 day storage of pooled platelets. The use of Composol as a platelet additive solution is to stabilize the pH of the final product during the 5 day of storage. This study was divided into 4 parts. In the 1st. part of this study, 20 pooled platelets were prepared. Initially 300ml. Composol were used for 5 buffy coats, but by trial and error it was determined that 100ml. Composol was the best amount to be used so that the final platelet count would be within the Council of Europe regulations. Therefore the remaining 3 parts of the study were analyzed by using 100ml. Composol for 5 buffy coats, each buffy coat having approximately the median weight within the range, that is 116g. In the 2nd part of the study, 38 pooled platelets were prepared and these were analysed for pH, pC02, and p02.from Dayl to day 7 of storage. The pH range should be between 6.4 to 7.4 according to the 'Guide to the preparation, use and quality assurance of blood c01nponents' regulations. In the 3rd. part of the study, 101 pooled platelets were prepared during the study period to analyze the Corrected Count Increment on patients after transfusion. Since only 43 pooled platelets were transfused between 1st. And 2nd. Day of preparation only these were calculated for the Corrected Count Increment. In the 4th. Part of this study, two types of comparisons were made with a previous study done by Agius in 2003. Agius had prepared pooled platelets by using plasma instead of Composol PS as an additive solution for platelets and therefore one can compare what are the advantages and disadvantages between the two systems were compared. The first comparison was done for the storage lesion by comparing pH, pC02, and p02 and the second comparison was carried out to determine the different values obtained when analyzing the Corrected Count Increments when using pooled platelets prepared with different products as an additive solution for platelets.