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https://www.um.edu.mt/library/oar/handle/123456789/77757| Title: | Cloning of Escherichia Coli iron superoxide dismutase in yeast cells deficient in copper, zinc superoxide dismutase |
| Authors: | Agius, Dorita |
| Keywords: | Escherichia coli Cloning Yeast |
| Issue Date: | 1996 |
| Citation: | Agius, D.R. (1996). Cloning of Escherichia Coli iron superoxide dismutase in yeast cells deficient in copper, zinc superoxide dismutase (Master's dissertation). |
| Abstract: | An episomal and a centromeric plasmid were used in the cloning of ‘Esherichia coli’ iron superoxide dismutase (FeSOD) in ‘Saccharomyces cervisiae’ cells deficient in copper, zinc superoxide dismutase (Cu,ZnSOD). In both cases the ‘E.coli’ FeSOD gene was unde the transcriptional control of the yeast PGK promoter. The prokaryotic FeSOD was shown to be functionally expressed at different levels within the two recombinant strains. Moreover, the FeSOD activity was found to decrease as the cells approached stationary phase in both strains. This decrease is attributed to the decrease in glucose concentration within the fixed volume medium. |
| Description: | M.PHIL. |
| URI: | https://www.um.edu.mt/library/oar/handle/123456789/77757 |
| Appears in Collections: | Dissertations - FacM&S - 1996 Dissertations - FacM&SPB - 1975-1996 |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| M.PHIL.Agius_Dolores _Rita_1996.pdf Restricted Access | 13.21 MB | Adobe PDF | View/Open Request a copy |
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